Tim Tolker-Nielsen has a Master of Science degree (chemical engineering) and a PhD degree (molecular microbiology) from the Technical University of Denmark, and a DMSc degree (biofilm infections) from the University of Copenhagen. His work has uncovered mechanisms of biofilm formation and dispersion, and the regulation of these processes via the signaling molecule c-di-GMP. Based on this knowledge he has developed anti-biofilm drug candidates that can force bacteria out of the biofilm state and into an antibiotic-susceptible single-cell state. In addition, his research has uncovered mechanisms of biofilm-associated antibiotic tolerance.